| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Neff, Alexander K (14)
|
VATIDE |
52.09 |
54.11 |
2.02 |
| 2 |
Lamoureux, Gavin L (14)
|
VATIDE |
53.49 |
54.84 |
1.35 |
| 3 |
Heritage, Barrett T (13)
|
VATIDE |
54.84 |
58.17 |
3.33 |
| 4 |
Younger, Cole C (14)
|
VACGBD |
57.27 |
59.42 |
2.15 |
| 5 |
Mateer, Cameron K (14)
|
VACGBD |
58.63 |
1:00.92 |
2.29 |
| 6 |
Muench, Hunter D (14)
|
VACGBD |
1:01.62 |
1:01.19 |
-0.43 |
| 7 |
Anderson, Seth E (14)
|
VATIDE |
1:05.88 |
1:02.17 |
-3.71 |
| 8 |
Barnes, Jonah A (14)
|
VAECAT |
1:10.65 |
1:02.77 |
-7.88 |
| 9 |
Whetstine, Clayton C (13)
|
VACGBD |
1:04.87 |
1:04.30 |
-0.57 |
| 10 |
Homer, Christopher E (14)
|
VACGBD |
1:04.04 |
1:04.85 |
0.81 |
| 11 |
Kinnear, Austin N (14)
|
VAECAT |
1:04.53 |
1:06.07 |
1.54 |
| 12 |
Hillery, Ryan P (13)
|
VATIDE |
1:01.77 |
1:06.11 |
4.34 |
| 13 |
Comeyne, Ethan C (14)
|
VATIDE |
1:05.57 |
1:06.74 |
1.17 |
| 14 |
Reed, John P (14)
|
VATIDE |
1:10.79 |
1:07.15 |
-3.64 |
| 15 |
Barrows, Nicholas W (13)
|
VACGBD |
1:03.72 |
1:08.04 |
4.32 |
| 16 |
Teer, Jacob A (13)
|
VACGBD |
1:07.80 |
1:08.99 |
1.19 |
| 17 |
Galvan, Edilberto A (14)
|
VACGBD |
1:07.94 |
1:14.77 |
6.83 |
| 18 |
Gervase, Philip R (13)
|
VAECAT |
|
1:17.34 |
- |
| 19 |
Smith, Ryan E (13)
|
VATIDE |
1:21.24 |
1:22.46 |
1.22 |